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codesniktoday at 2:10 PM1 replyview on HN

pcr amplifies all sequences, correct or wrong, no? and as I understand it, it works on short snippets the best.


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biophysboytoday at 2:21 PM

It amplifies sequences that contain the two primer sequences on each end of the target. So if you had synthesized sequence XYZ with some mistakes like YZX, then you could target X and Z and purify.

You're correct that PCR has a limited max length, but it is longer and cheaper than vanilla DNA synthesis.

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