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oofbeytoday at 3:20 PM1 replyview on HN

Intuitively I agree some kind of selective amplification should be able to correct for the mistakes. But I think it will be complicated. Because the filtering process needs to be much more complex. It can’t just chemically match to a known subsequence - you won’t know where the mistake might be in a long sequence.


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biophysboytoday at 4:25 PM

This is a good point. WXYZ and WYXZ are indistinguishable via PCR. And the possibilities accumulate with more segments.